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宸墿閺＄浉鍚岋紝浣挎槧鍍忔竻鏅�銆佸钩鍧︼紱浣嗙祼(ji茅)妲�(g貌u)寰�(f霉)闆�锛屽埗閫犲洶闆ｃ��
鈷屽崐寰�(f霉)娑堣壊宸墿閺★紙Halfapochromatic) 閮ㄥ垎閺＄墖鐢ㄧ啋鐭冲埗鎴�锛屾晠鍙堢ū鐔掔煶鐗╅彙锛屾�ц兘姣旀秷鑹插樊鐗╅彙濂�锛�
鍍规牸姣斿京(f霉)娑堣壊宸墿閺′究瀹溿�傛牎姝ｅ儚宸▼搴︿粙浜庢秷鑹插樊鑸囧京(f霉)娑堣壊宸叐绋墿閺′箣闁�锛屼絾鍏跺畠鍏夊(xu茅)鎬ц唱(zh矛)閮借垏鍚庤��
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1锛夌浉宸墿閺★紙phase contrast objective锛夐�欑ó鐗╅彙鏄浉宸’寰彙鐨勫皥鐢ㄩ彙闋紙鐣�(d膩ng)鐒朵篃鍙父瑕�(gu墨)浣跨敤锛夈��
鐗归粸鏄湪鐗╅彙鐨勫悗鐒﹂粸骞抽潰铏曡鏈変竴濉婄浉鏉�锛屽凡閬�(d谩)鍒版帹閬插厜娉㈢殑鐩殑銆�
2锛夊付鏍℃鐠�(hu谩n)鐗╅彙锛坈orrection collar objective锛夊湪鐗╅彙涓鏈夌挵(hu谩n)鐙�鐨勮(di脿o)绡�(ji茅)鐠�(hu谩n)銆傜暥(d膩ng)杞�(zhu菐n)鍕曡(di脿o)绡�(ji茅)鐠�(hu谩n)鏅傦紝鍙(di脿o)
绡�(ji茅)鐗╅彙鍏�(n猫i)鐨勯�忛彙绲勶紙涓�鑸偤绗簩鍜岀涓夌祫閫忛彙锛変箣闁撶殑璺濋洟锛屽緸鑰屾牎姝ｇ敱钃嬬幓鐗囧帤搴︿笉妯�(bi膩o)婧�(zh菙n)鎵�寮曡捣鐨勮钃�
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3锛夊付铏瑰僵鍏夐棇鐗╅彙锛坕ris diaphragm objective锛夊湪鐗╅彙閺＄瓛鍏�(n猫i)鐨勪笂閮ㄨ鏈夎櫣褰╁厜闂�锛屽鏂逛篃鏈夊彲鏃嬭綁(zhu菐n)
鐨勮(di脿o)绡�(ji茅)鐠�(hu谩n)锛岃綁(zhu菐n)鍕曟檪鍙(di脿o)绡�(ji茅)鍏夐棇瀛斿緫鐨勫ぇ灏�銆傞�欑ó绲�(ji茅)妲�(g貌u)鏄殑娌规蹈鐗╅彙銆�
4锛夌劇鎳�(y墨ng)璁婄墿閺★紙strain-free objective锛夐�欑ó鐗╅彙鍦ㄩ�忛彙绲勭殑瑁濋厤涓厠鏈嶄簡鎳�(y墨ng)鍔涚殑瀛樺湪锛屾槸灏堜綔閫忓皠寮�
鍋忓厜閺℃鐢ㄧ殑鐗╅彙锛岃兘閬�(d谩)鍒版洿浣崇殑鍋忓厜閺℃鏁堟灉銆�
5锛夌劇鐔掑厜鐗╅彙锛坣on-fluorescing objective锛夋槸灏堢敤浜庤惤灏勫紡鐔掑厜椤井閺′笂鐨勭墿閺�銆傞�欑ó鐗╅彙鍗充娇鍙楀埌
寰堝挤鐨勬縺鍕靛厜婧愭檪涔熶笉鐧�(f膩)鍑虹啋鍏�銆�
6锛夌劇钃嬬墖鐗╅彙锛坣o cover objective锛� 鏈変簺琚鐗╅珨锛屽挨鍏舵槸娑傛姽鍒剁墖绛�锛屼笂闈笉鑳藉姞鐢ㄨ搵鐜荤墖锛岄�欐ǎ
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鍦ㄨ桓鑹插樊鏍℃涓�锛屾牎姝ｄ簡C绶氾紙绱咃細656.3 nm锛夊拰F绶氾紙钘�(l谩n)锛�486.1 nm锛�2绋鑹茬殑鐗╅彙绋辩偤娑堣壊宸�忛彙锛圓chromat锛夈�傜磪钘�(l谩n)2鑹蹭互澶栫殑鍏夌窔锛堜竴鑸互绱壊鐨刧绶氱偤灏嶈薄锛�435.8 nm锛夊湪闆㈤枊鐒﹀钩闈㈢殑闈笂鑱氱劍锛岄�欏�媑绶氱ū鐐�2绱氬厜璀溿�傝壊宸牎姝ｈ寖鍦嶉仈(d谩)鍒伴�欏��2绱氬厜璀滅殑鐗╅彙绋辩偤寰�(f霉)娑堣壊宸�忛彙锛圓pochromat锛�銆備篃灏辨槸瑾紝寰�(f霉)娑堣壊宸�忛彙鏄皪3鑹诧紙C绶�銆丗绶氥�乬绶氾級閫�(j矛n)琛岃桓鑹插樊鏍℃鐨勭墿閺�銆備笅鍦栦互娉㈠儚宸〃绀轰簡娑堣壊宸�忛彙鍜屽京(f霉)娑堣壊宸�忛彙鍦ㄨ壊宸牎姝ｄ笂鐨勪笉鍚�銆傜敱姝ゅ湒鍙互鐪嬪嚭锛岃垏娑堣壊宸�忛彙鐩告瘮锛屽京(f霉)娑堣壊宸�忛彙鍙互鍦ㄦ洿寤ｇ殑娉㈤暦鑼冨湇鍏�(n猫i)鏍℃鑹插樊銆�
鑹插樊鏍℃鐨勬瘮杓冿紙娑堣壊宸�忛彙鍜屽京(f霉)娑堣壊宸�忛彙锛�
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鐣�(d膩ng)鐢ㄦ櫘閫氱殑涓ぎ鐓ф槑娉曪紙浣垮厜绶氬潎鍕诲湴閫忛亷妯�(bi膩o)鏈殑鏄庤鐓ф槑娉曪級鏅�锛岄’寰彙鐨勫垎杈ㄨ窛闆㈢偤d=0.61位/NA寮忎腑d鈥斺�旂墿閺＄殑鍒嗚鲸璺濋洟锛屽柈浣� nm銆偽烩�斺�旂収鏄庡厜绶氭尝闀�锛屽柈浣� nm銆侼A 鈥斺�旂墿閺＄殑鏁�(sh霉)鍊煎瓟寰�锛屼緥濡傛补娴哥墿閺＄殑鏁�(sh霉)鍊煎瓟寰戠偤1.25锛屽彲瑕嬪厜娉㈤暦鑼冨湇鐐�400鈥�700nm 锛屽彇鍏跺钩鍧囨尝闀�550 nm锛屽墖d=270 nm锛岀磩绛変簬鐓ф槑鍏夌窔娉㈤暦涓�鍗�銆備竴鑸湴锛岀敤鍙鍏夌収鏄庣殑椤井閺″垎杈ㄥ姏鐨勬サ闄愭槸0.2渭m銆�
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