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    Bradford 娉曪細閫欑ó鏂规硶鐨勫師鐞嗘槸铔嬬櫧璩�(zh矛)鑸囪�冮Μ鏂�浜�铇�绲�(ji茅)鍚堝弽鎳�(y墨ng)锛岀敘(ch菐n)鐢熺殑鏈夎壊鍖栧悎鐗╁惛鏀跺嘲595nm銆傚叾鏈�澶х殑鐗归粸(di菐n)鏄�锛屾晱鎰熷害濂�锛屾槸Lowry 鍜孊CA 鍏╃ó娓�瑭︽柟娉曠��2 鍊�锛涙搷浣滄洿绨″柈锛岄�熷害鏇村揩锛涘彧闇�瑕佷竴绋�鍙嶆��(y墨ng)瑭﹀姂锛涘寲鍚堢墿鍙�浠ョ��(w臎n)瀹�1灏忔檪(sh铆)锛屾柟渚跨祼(ji茅)鏋�锛涜�屼笖鑸囦竴绯诲垪骞叉摼Lowry锛孊CA 鍙嶆噳(y墨ng)鐨勯倓鍘熷姂锛堝�侱TT锛屽钒鍩轰箼閱囷級鐩稿�广�備絾鏄�灏嶄簬鍘绘薄鍔戜緷鐒舵槸鏁忔劅鐨�銆傛渶涓昏�佺殑缂洪��(di菐n)鏄�涓嶅悓鐨勬��(bi膩o)婧�(zh菙n)鍝佹渻灏�(d菐o)鑷村悓涓�妯ｅ搧鐨勭祼(ji茅)鏋滃樊鐣拌純澶э紝鐒″彲姣旀��銆�
    鏌愪簺鍒濇�℃帴瑙告瘮鑹叉硶娓�瀹氱殑鐮旂┒鑰呭彲鑳界偤鍚勭ó姣旇壊娉曟脯鍑虹殑绲�(ji茅)鏋滃苟涓嶄竴鑷�锛屾劅鍒拌糠鎯�锛岀┒绔熻┎鐩镐俊鍝�绋�鏂规硶锛熺敱浜庡悇绋�鏂规硶鍙嶆��(y墨ng)鐨勫熀鍦�(tu谩n)浠ュ強椤�鑹插熀鍦�(tu谩n)涓嶄竴锛屾墍浠ュ悓鏅�(sh铆)浣跨敤骞剧ó鏂规硶灏嶅悓涓�妯ｅ搧寰楀嚭鐨勬ǎ鍝佹績搴︾劇鍙�姣旀�с�備緥濡傦細Keller绛夋脯瑭︿汉濂朵腑鐨勮泲鐧�锛岀祼(ji茅)鏋淟owry锛孊CA 娓�鍑虹殑婵冨害鏄庨’楂樹簬Bradford锛屽樊鐣伴’钁�銆傚嵆浣挎槸娓�瀹氬悓涓�妯ｅ搧锛屽悓涓�绋�姣旇壊娉曢伕鎿囩殑妯�(bi膩o)婧�(zh菙n)妯ｅ搧涓嶄竴鑷�锛屾脯瑭﹀悗鐨勬績搴︿篃涓嶄竴鑷�銆傚�傜敤Lowry娓�瑭︾��(x矛)鑳炲嫽婕夸腑鐨勮泲鐧借唱(zh矛)锛屼互BSA浣滄��(bi膩o)婧�(zh菙n)鍝�锛屾績搴�1.34mg/ml锛屼互a鐞冭泲鐧戒綔妯�(bi膩o)婧�(zh菙n)鍝侊紝婵冨害2.64mg/ml銆傚洜姝�锛屽湪閬告搰姣旇壊娉曚箣鍓嶏紝鏈�濂芥槸鍙冪収瑕佹脯瑭︾殑妯ｆ湰鐨勫寲瀛�(xu茅)妲�(g貌u)鎴�锛屽皨鎵惧寲瀛�(xu茅)妲�(g貌u)鎴愰�炰技鐨勬��(bi膩o)婧�(zh菙n)铔嬬櫧浣滄��(bi膩o)婧�(zh菙n)鍝�銆傚彟澶栵紝姣旇壊娉曞畾閲忚泲鐧借唱(zh矛)锛岀稉(j墨ng)甯稿嚭鐝�(xi脿n)鐨勫晱椤屾槸妯ｅ搧鐨勫惛鍏夊�煎お浣庯紝灏�(d菐o)鑷存脯鍑虹殑妯ｅ搧婵冨害鑸囧��(sh铆)闅涚殑婵冨害宸�璺濊純澶�銆傞棞(gu膩n)閸靛晱椤屾槸锛屽弽鎳�(y墨ng)鍚�1011鍒嗗厜鍏夊害瑷�(j矛)鐨勯噸瑕侀厤浠垛�斺�� 姣旇壊鏉�鐨勯�忚壊鏄�鏈変竴瀹氱殑鍗婅“鏈�锛屾墍浠ユ瘡绋�姣旇壊娉曢兘鍒楀嚭浜嗗弽鎳�(y墨ng)娓�瑭︽��(sh铆)闁�锛屾墍鏈夌殑妯ｅ搧锛堝寘鎷�妯�(bi膩o)婧�(zh菙n)妯ｅ搧锛�锛岄兘蹇呴爤鍦ㄦ�ゆ��(sh铆)闁撳収(n猫i)娓�瑭︺�傛檪(sh铆)闁撻亷闀�锛屽緱鍒扮殑鍚稿厜鍊艰畩灏忥紝鎻涚畻鐨勬績搴﹀�奸檷浣�銆傞櫎姝わ紝鍙嶆噳(y墨ng)婧�搴�銆佹憾娑睵H鍊肩瓑閮芥槸褰遍熆瀵�(sh铆)椹�(y脿n)鐨勯噸瑕佸師鍥�銆傛�ゅ��锛岄潪甯搁噸瑕佺殑鏄�锛屾渶濂芥槸鐢ㄥ�戞枡鐨勬瘮鑹叉硶銆傞伩鍏嶄娇鐢ㄧ煶鑻辨垨鑰呯幓鐠冩潗璩�(zh矛)鐨勬瘮鑹叉澂锛屽洜?y脿n)姒夌；?y墨ng)鍚庣殑椤忚壊鏈冭畵鐭宠嫳鎴栬�呯幓鐠冭憲鑹�锛屽皫(d菐o)鑷存ǎ鍝佸惛鍏夊�间笉婧�(zh菙n)纰恒��
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